Subject:Info from UCLA – Andrew Norris
Date:Thursday, March 31, 2011 1:37:00 PM
I am sifting through public inquiries and see one from Andrew Norris at the Department of Radiation Oncology in LA. Enclosed is a docu~ment entitled, “Radioprotective Properties of Tetracycline.” I thought you may be interested and wanted to see if I should drop it off
External data from MD Anderson Cancer Research Center
High-throughput screening identifies two classes of antibiotics as radioprotectors: Tetracyclines and fluoroquinolones
Kwanghee Kim; Julianne M. Pollard; Andrew J. Norris; J. Tyson McDonald; Yingli Sun; Ewa Micewicz; Kelly Pettijohn; Robert Damoiseaux; Keisuke S. Iwamoto et al (Profiled Author: Julianne M. Pollard)
Clinical Cancer Research 2009;15(23):7238-7245.
Purpose: Discovery of agents that protect or mitigate normal tissue from radiation injury during radiotherapy, accidents, or terrorist attacks is of importance. Specifically, bone marrow insufficiency, with possible infection due to immunosuppression, can occur after total body irradiation (TBI) or regional irradiation and is a major component of the acute radiation syndrome. The purpose of this study was to identify novel radioprotectors and mitigators of the hematopoietic system. Experimental Design: High-throughput screening of small-molecule libraries was done using viability of a murine lymphocyte line as a readout with further validation in human lymphoblastoid cells. The selected compounds were then tested for their ability to counter TBI lethality in mice. Results: All of two major classes of antibiotics, tetracyclines and fluoroquinolones, which share a common planar ring moiety, were radioprotective. Furthermore, tetracycline protected murine hematopoietic stem/progenitor cell populations from radiation damage and allowed 87.5% of mice to survive when given before and 35% when given 24 h after lethal TBI. Interestingly, tetracycline did not alter the radiosensitivity of Lewis lung cancer cells. Tetracycline and ciprofloxacine also protected human lymphoblastoid cells, reducing radiation-induced DNA double-strand breaks by 33% and 21%, respectively. The effects of these agents on radiation lethality are not due to the classic mechanism of free radical scavenging but potentially through activation of the Tip60 histone acetyltransferase and altered chromatin structure. Conclusions: Tetracyclines and fluoroquinolones can be robust radioprotectors and mitigators of the hematopoietic system with potential utility in anticancer radiotherapy and radiation emergencies. © 2009 American Association for Cancer Research.